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KMID : 0613820170270070754
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Journal of Life Science
2017 Volume.27 No. 7 p.754 ~ p.759
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Optimized Processing Condition of Production of Nannochloropsis oculata under Light-emitting Diode (LED) Condition
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Lee Nam-Kyu
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Abstract
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The 100 l culture system was made on the basis of LED light, and Nannochloropsis oculata was cultured in f/2 medium at light intensity (100 μmol/m2/s), culture temperature (20℃±1℃) and LD cycle (12 hr). As a result, the maximum biomass of 1.07 g/l was cultured as a result of 100 l mass culture at 100 μmol/m2/s and 24 mg/l nitrate concentration in LED blue (475 nm). The extraction was carried out using sonicator, homogenizer and chemical method 0.5M HCl shredding method. The contents of chlorophyll a, chlorophyll b and carotenoid were 1.6, 0.5 and 0.3 mg/g cell. When using homogenizer, it was measured at 1.0, 0.6 and 0.2 mg/g cell. The chemical breakdown method of 0.5M HCl, chlorophyll a, b, and carotenoid contents were measured as 0.9, 0.8, 0 mg/g cell. The highest amount of biomass during the distruption time was measured at 3.6 mg/g cell at 15 min disintegration and acetone, 3.6 mg/g cell of acetone, methanol, and ethanol were measured as effective solvents. Concentration was measured by using microfilter, disk type continuous centrifuge and tubular type continuous centrifuge were 16.0, 1.1 and 0.5 g/l, respectively. Four kinds of equipment such as hot air dryer, vacuum dryer, spray dryer and freeze dryer were tested to optimize the drying process. As a result, the recovery rates of spray dryer and freeze dryer were 80% and 60%.
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KEYWORD
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Distruption time, freeze dryer, light intensity, microfilter, Nannochloropsis oculata
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